1F99
CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1997-12-10 |
| Detector | WEISSENBERG |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 135.100, 135.100, 210.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.375 |
| Total number of observations | 266953 * | |
| Number of reflections | 73674 | |
| Completeness [%] | 75.5 | 49 * |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | The hanging-drop contains 6mg/ml R-phycocyanin, 0.05M Na2HPO4/NaH2PO4 and (NH4)2SO4 (pH7.0). The diffusion buffer contained 10%-20% (NH4)2SO4 and 10% NaCl, 0.05M Na2HPO4/NaH2PO4, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | |
| 3 | 1 | drop | ammonium phosphate | 0.05 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 10-20 (%) | |
| 5 | 1 | reservoir | 10 (%) | ||
| 6 | 1 | reservoir | sodium phosphate | 0.05 (M) |
