1F8X

CRYSTAL STRUCTURE OF NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	296
Detector technology	AREA DETECTOR
Collection date	1989-01-01
Detector	SIEMENS-NICOLET X100
Spacegroup name	I 21 3
Unit cell lengths	151.000, 151.000, 151.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.500
Rwork	0.166
R-free	0.24000
RMSD bond length	0.007
RMSD bond angle	1.540
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	PHASES
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.500
Rmerge	0.115
Number of reflections	19875
Completeness [%]	95.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	296	ammonium sulfate, citrate buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	5.4	296	ammonium sulfate, citrate buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	5.4	296	ammonium sulfate, citrate buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	ammonium sulfate	5 (%sat)
3	1	drop	citrate	0.05 (M)
4	1	reservoir	ammonium sulfate	5 (%sat)
5	1	reservoir	citrate	0.05 (M)