1F8T
FAB (LNKB-2) OF MONOCLONAL ANTIBODY, CRYSTAL STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.240, 72.010, 86.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.200 |
| Rwork | 0.171 |
| R-free | 0.22100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.194 |
| Number of reflections | 21459 | |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 90.6 | 94.2 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | PEG-4000, 2-propanol, Na-citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
