1F8S

CRYSTAL STRUCTURE OF L-AMINO ACID OXIDASE FROM CALLOSELASMA RHODOSTOMA, COMPLEXED WITH THREE MOLECULES OF O-AMINOBENZOATE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	83
Detector technology	AREA DETECTOR
Collection date	1999-08-31
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	77.553, 137.180, 212.634
Unit cell angles	90.00, 105.63, 90.00

Refinement procedure

Resolution	50.000 * - 2.000
R-factor	0.205 *
Rwork	0.205
R-free	0.22500
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.083	0.378
Number of reflections	335974
<I/σ(I)>	11.7
Completeness [%]	85.0	47.4
Redundancy	3.5	1.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	Jancarik, J., (1991) J. Appl. Crystallogr., 24, 409. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)