1F8R

CRYSTAL STRUCTURE OF L-AMINO ACID OXIDASE FROM CALLOSELASMA RHODOSTOMA COMPLEXED WITH CITRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	83
Detector technology	AREA DETECTOR
Collection date	1998-08-16
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	79.496, 154.962, 102.947
Unit cell angles	90.00, 109.49, 90.00

Refinement procedure

Resolution	50.000 * - 2.000
R-factor	0.185 *
Rwork	0.185
R-free	0.21000
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.159
Total number of observations	391000 *
Number of reflections	139242
<I/σ(I)>	18.9
Completeness [%]	88.1	71.7
Redundancy	2.8	2.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	Jancarik, J., (1991) J. Appl. Crystallogr., 24, 409. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)