1F8N
LIPOXYGENASE-1 (SOYBEAN) AT 100K, NEW REFINEMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1994-02-01 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.900, 94.000, 49.900 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.21200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.037 | 0.343 |
| Number of reflections | 161029 | |
| <I/σ(I)> | 35.7 | |
| Completeness [%] | 96.0 | 91.6 |
| Redundancy | 6.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 * | 21 * | Minor, W., (1996) Biochemistry, 35, 10687. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | acetate | 0.1 (M) | |
| 2 | 1 | drop | PEG3400 | 4 (%(w/v)) | |
| 3 | 1 | drop | protein | 4 (mg/ml) | |
| 4 | 1 | reservoir | PEG3350 | 9 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.2 (M) |
