1F8B

Native Influenza Virus Neuraminidase in Complex with NEU5AC2EN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-12-20
Detector	RIGAKU RAXIS II
Spacegroup name	I 4 3 2
Unit cell lengths	181.030, 181.030, 181.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.800
Rwork	0.166
R-free	0.21400
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.071
Number of reflections	30479
<I/σ(I)>	10.6
Completeness [%]	75.0	92.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.9	293	Laver, W.G., (1984) Virology, 137, 314. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	1.7 (M)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	potassium phosphate	1.9 (M)