1F8A

STRUCTURAL BASIS FOR THE PHOSPHOSERINE-PROLINE RECOGNITION BY GROUP IV WW DOMAINS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-15
Detector	MACSCIENCE DIP100S
Spacegroup name	P 21 21 21
Unit cell lengths	35.270, 43.903, 124.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.410 - 1.840
R-factor	0.231
Rwork	0.231
R-free	0.27100
RMSD bond length	0.029
RMSD bond angle	2.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.020	1.900
High resolution limit [Å]	1.840	1.840
Rmerge	0.062	0.337
Total number of observations	293095 *
Number of reflections	17107 *
<I/σ(I)>	21.5
Completeness [%]	97.3	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	100 mM MOPSO-Na+, 28% PEG 8000, 2 mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MOPSO-Na+	100 (mM)
2	1	reservoir	PEG8000	28 (%(v/v))
3	1	reservoir	dithiothreitol	2 (mM)