1F88
CRYSTAL STRUCTURE OF BOVINE RHODOPSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-12 |
| Detector | SBC-2 |
| Spacegroup name | P 41 |
| Unit cell lengths | 97.246, 97.246, 149.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.186 |
| R-free | 0.23800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.121 | 0.690 |
| Total number of observations | 111245 * | |
| Number of reflections | 33221 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 97.0 | 80.7 |
| Redundancy | 3.35 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | Okada, T., (2000) J. Struct. Biol., 130, 73. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 30 (mM) | or sodium acetate |
| 2 | 1 | reservoir | beta-mercaptoehtanol | 5-7 (mM) | |
| 3 | 1 | reservoir | 65-90 (mM) | ||
| 4 | 1 | reservoir | HPTO | 0.55-0.75 (%) | |
| 5 | 1 | reservoir | NG | 0.45-0.55 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.84-0.86 (M) |
