1F86

TRANSTHYRETIN THR119MET PROTEIN STABILISATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	42.680, 85.770, 63.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.100
R-factor	0.138 *
Rwork	0.147
R-free	0.17700
Structure solution method	WILD-TYPE TTR (1TTA)
RMSD bond length	0.015
RMSD bond angle	0.037
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.110
High resolution limit [Å]	1.100	1.100
Rmerge	0.048	0.497
Total number of observations	1561421 *
Number of reflections	95607 *
<I/σ(I)>	25.1
Completeness [%]	98.4	95.3
Redundancy	16	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3 *	277	Damas, A.M., (1996) Acta Crystallog., D52, 966. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	41 (%)
3	1	reservoir	sodium citrate	200 (mM)