1F80
HOLO-(ACYL CARRIER PROTEIN) SYNTHASE IN COMPLEX WITH HOLO-(ACYL CARRIER PROTEIN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.458, 122.032, 136.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f7t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.057 | 0.560 |
| Total number of observations | 270151 * | |
| Number of reflections | 29694 * | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 97.7 * | 94.5 |
| Redundancy | 9.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.4 | 291 | protein: 50mM Bis-Tris pH 6.4, 100mM sodium chloride, 10mM magnesium chloride, 10mM dithiothreitol Well: 0.15-0.3M potassium formate, 15-23% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | potassium formate | 0.15-0.3 (M) | |
| 3 | 1 | 2 | PEG3350 | 15-23 (%) | |
| 4 | 1 | 1 | Bis-Tris | 50 (mM) | pH6.4 |
| 5 | 1 | 1 | 100 (mM) | ||
| 6 | 1 | 1 | 10 (mM) | ||
| 7 | 1 | 1 | dithiothreitol | 10 (mM) |
