1F7S

CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 2 2 21
Unit cell lengths	55.873, 71.308, 63.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.000
R-factor	0.211 *
Rwork	0.210
R-free	0.24800
RMSD bond length	0.005
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.800	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.045	0.135
Total number of observations	60228 *
Number of reflections	8926
<I/σ(I)>	15.6
Completeness [%]	98.9	99 *
Redundancy	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6 *	4 *	Ammonium sulfate, sodium citrate, LDAO, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	4 (mM)
2	1	drop	dithiothreitol	0.4 (mM)
3	1	drop	protein	7.2-10 (mg/ml)
4	1	drop	ammonium sulfate	0.8 (M)
5	1	drop	citrate	50 (mM)
6	1	drop	LDAO	2 (mM)
7	1	reservoir	ammonium sulfate	1.6 (M)
8	1	reservoir	citrate	100 (mM)