1F7C
CRYSTAL STRUCTURE OF THE BH DOMAIN FROM GRAF, THE GTPASE REGULATOR ASSOCIATED WITH FOCAL ADHESION KINASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.026, 65.026, 91.624 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.205 |
R-free | 0.25200 |
Structure solution method | MIR and MR |
RMSD bond length | 0.006 |
RMSD bond angle | 19.400 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.077 | 0.330 * |
Total number of observations | 30884 * | |
Number of reflections | 9020 * | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.0 | 98 * |
Redundancy | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | PEG 6000, Na HEPES, pH 7.0, VAPOR DIFFUSION, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 20-22 (%) | |
3 | 1 | reservoir | HEPES | 100 (mM) |