1F75

CRYSTAL STRUCTURE OF UNDECAPRENYL DIPHOSPHATE SYNTHASE FROM MICROCOCCUS LUTEUS B-P 26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-02-04
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	127.197, 60.161, 75.703
Unit cell angles	90.00, 105.69, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.190
R-free	0.24600
Structure solution method	MIRAS
RMSD bond length	0.006 *
RMSD bond angle	1.120 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.032	0.168
Total number of observations	57323 *
Number of reflections	23530 *
<I/σ(I)>	24.3
Completeness [%]	83.7	56.8
Redundancy	2.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	293	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	sodium citrate	0.1 (M)
6	1	reservoir	ammonium sulfate	0.5 (M)
7	1	reservoir	lithium sulfate	1.0 (M)