1F6O
CRYSTAL STRUCTURE OF THE HUMAN AAG DNA REPAIR GLYCOSYLASE COMPLEXED WITH DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-06 |
Detector | PHILLIPS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.750, 57.100, 128.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 2.400 |
Rwork | 0.219 |
R-free | 0.28200 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.071 | 0.253 |
Total number of observations | 100868 * | |
Number of reflections | 13252 * | |
<I/σ(I)> | 21.5 | |
Completeness [%] | 86.1 * | 75.6 |
Redundancy | 8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 * | 298 | PEG 8000, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | protein | 0.3 (mM) | ||
2 | 1 | sodium chloride | 100 (mM) | ||
3 | 1 | HEPES | 20 (mM) | ||
4 | 1 | 1 | EDTA | 0.1 (mM) | |
5 | 1 | 1 | glycerol | 5 (%) |