1F6N

CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT REACTION CENTER PRO L209-> TYR FROM THE PHOTOSYNTHETIC PURPLE BACTERIUM RHODOBACTER SPHAEROIDES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1998-07-08
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	141.535, 141.535, 187.913
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.221
Rwork	0.221
R-free	0.25100 *
RMSD bond length	0.010
RMSD bond angle	24.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.059	0.270
Number of reflections	52164
<I/σ(I)>	8.9
Completeness [%]	96.0	95.4
Redundancy	1.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	18 *	Fritzsch, G.,(1998) Methods Enzymol., 297, 57. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop		4 (M)	composed of 20mM NaH2PO4, 0.063%LDAO, 1.5M (NH4)2SO4, 3% 1,2,3-heptanetriol, and RC with an OD830=25.
3	1	reservoir		1.8-2.1 (M)