1F6F
CRYSTAL STRUCTURE OF THE TERNARY COMPLEX BETWEEN OVINE PLACENTAL LACTOGEN AND THE EXTRACELLULAR DOMAIN OF THE RAT PROLACTIN RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.180, 63.100, 88.430 |
| Unit cell angles | 90.00, 118.63, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| Rwork | 0.225 |
| R-free | 0.28700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.610 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.130 |
| Number of reflections | 33034 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 90.7 | 70.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | Christinger, H.W., (1998) Acta Crystallogr., Sect.D, 54, 1408. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%) | |
| 2 | 1 | reservoir | 2-propanol | 15 (%) | |
| 3 | 1 | reservoir | MPD | 1 (%) | |
| 4 | 1 | reservoir | MES | 0.1 (M) |
