1F5P

2.9 ANGSTROM CRYSTAL STRUCTURE OF LAMPREY HEMOGLOBIN THAT HAS BEEN EXPOSED TO CARBON MONOXIDE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	296
Detector technology	IMAGE PLATE
Collection date	2000-03-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	82.500, 96.200, 145.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.900
R-factor	0.23 *
Rwork	0.206
R-free	0.25400 *
RMSD bond length	0.008 *
RMSD bond angle	1.586
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.118 *	0.375 *
Total number of observations	81837 *
Number of reflections	24593 *
<I/σ(I)>	7.4
Completeness [%]	93.9 *	79.3 *
Redundancy	3.01	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.8	296	25% PEG 4K, 170mM phosphate buffer, pH 6.8, SMALL TUBES, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	25 (%)
2	1	1	phosphate	170 (mM)