1F5N

HUMAN GUANYLATE BINDING PROTEIN-1 IN COMPLEX WITH THE GTP ANALOGUE, GMPPNP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-16
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	161.228, 42.652, 91.544
Unit cell angles	90.00, 100.19, 90.00

Refinement procedure

Resolution	41.190 - 1.700
R-factor	0.226
Rwork	0.226
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1DG3 with some deletions
RMSD bond length	0.005
RMSD bond angle	19.400 *
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.190	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.084	0.251
Number of reflections	67508
<I/σ(I)>	12.05
Completeness [%]	99.2	74 *
Redundancy	5.22	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	15-20% PEG3350, 150-200mM MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 4K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	15-20 (%)
2	1	reservoir		150-200 (mM)