1F5F
CRYSTAL STRUCTURE OF THE N-TERMINAL G-DOMAIN OF SHBG IN COMPLEX WITH ZINC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.020, 104.020, 84.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.25900 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.035 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.287 |
| Total number of observations | 101085 * | |
| Number of reflections | 18785 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 96.3 | 93.3 * |
| Redundancy | 5.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 * | Grishkovskaya, I., (1999) Acta Crystallogr. Sec., D55, 2053 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | |
| 3 | 1 | drop | 2.5 (mM) | ||
| 4 | 1 | drop | DHT | 0.003 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 20 (%) | |
| 6 | 1 | reservoir | PEG400 | 10 (%) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) |
