1F50

BACTERIORHODOPSIN-BR STATE OF THE E204Q MUTANT AT 1.7 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-05
Detector	ADSC QUANTUM
Spacegroup name	P 63
Unit cell lengths	60.631, 60.631, 108.156
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 1.700
Rwork	0.132
R-free	0.19100
Starting model (for MR)	ISOMORPHOUS TO 1C3W model
RMSD bond length	0.009
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.057	0.296
Total number of observations	257539 *
Number of reflections	25709
<I/σ(I)>	29.6	2.9
Completeness [%]	99.1
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.6	20 *	Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. *
1	Batch method *	5.6	20 *	Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. *
1	Batch method *	5.6	20 *	Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	lipids	60-70 (%(w/v))
2	1	1	BR	2.5-4.5 (mg/ml)
3	1	1	sodium potassium phosphate	0.7-4.0 (M)
4	1	1	MPD	1.5-3.75 (%)
5	1	1	OG	0.36-0.48 (%)