1F4J

STRUCTURE OF TETRAGONAL CRYSTALS OF HUMAN ERYTHROCYTE CATALASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-26
Detector	RIGAKU RAXIS II
Spacegroup name	I 41
Unit cell lengths	203.600, 203.600, 144.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	144.000 * - 2.400
R-factor	0.196 *
Rwork	0.196
R-free	0.24400
RMSD bond length	0.008 *
RMSD bond angle	1.450 *
Data reduction software	bioteX
Data scaling software	bioteX
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.000 *	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.068	0.249
Total number of observations	337583 *
Number of reflections	107950	10206 *
<I/σ(I)>	7.8
Completeness [%]	92.3	76.4
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7 *	298	Ko, T.P., (2000) Acta Crystallogr., Sect.D, 56, 241. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	potassium sodium tartrate	0.7 (M)
3	1	reservoir	HEPES	0.1 (M)