1F4H

E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS
Synchrotron site	SRS
Spacegroup name	P 21 21 21
Unit cell lengths	153.400, 173.400, 204.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.137 *
Rwork	0.137
R-free	0.27900
RMSD bond length	0.016
RMSD bond angle	2.800
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.096	0.321 *
Total number of observations	299596 *
Number of reflections	116158
Completeness [%]	88.3 *	71
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	298	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	10 (%)
3	1	reservoir	Bis-Tris	100 (mM)
4	1	reservoir		200 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir	dithiothreitol	10 (mM)