1F47

THE BACTERIAL CELL-DIVISION PROTEIN ZIPA AND ITS INTERACTION WITH AN FTSZ FRAGMENT REVEALED BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-12-12
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	36.526, 38.901, 54.541
Unit cell angles	90.00, 104.11, 90.00

Refinement procedure

Resolution	25.000 - 1.950
R-factor	0.205
Rwork	0.205
R-free	0.25100
RMSD bond length	0.008
RMSD bond angle	1.420
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.055	0.146
Number of reflections	10556
<I/σ(I)>	26.9
Completeness [%]	95.6	88.6
Redundancy	3.92	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	291	30% PEG 6000, 100 mM Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG6000	25 (%)
3	1	reservoir	MES	100 (mM)