1F44
CRYSTAL STRUCTURE OF TRIMERIC CRE RECOMBINASE-LOX COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-06 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 160.653, 160.653, 160.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.050 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.22400 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.044 | 0.334 * |
| Number of reflections | 42556 | |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 98.6 | 99.9 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.1 | 20-22 * | 125 mM LI2SO4, 4% PEG 8000, 40mM NaPIPES PH 6.1, 5% MPD, VAPOR DIFFUSION, HANGING DROP at 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | LI2SO4 | ||
| 2 | 1 | 1 | NaPIPES | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 4 (%(w/v)) | |
| 2 | 1 | reservoir | 125 (mM) | ||
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | 60 (mM) |
