1F42
THE P40 DOMAIN OF HUMAN INTERLEUKIN-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.000, 55.000, 189.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.28100 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.880 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.054 | 0.160 |
| Total number of observations | 90266 * | |
| Number of reflections | 12537 * | |
| <I/σ(I)> | 35 | |
| Completeness [%] | 97.0 | 79 |
| Redundancy | 7.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | 18% PEG8000, 0.2M Calcium Acetate, 0.1M Na Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 18 (%) | |
| 4 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
