1F3T
CRYSTAL STRUCTURE OF TRYPANOSOMA BRUCEI ORNITHINE DECARBOXYLASE (ODC) COMPLEXED WITH PUTRESCINE, ODC'S REACTION PRODUCT.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 1999-11-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.294, 151.599, 86.414 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.237 * |
| Rwork | 0.237 |
| R-free | 0.28000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 8.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 * | 0.319 |
| Total number of observations | 402763 * | |
| Number of reflections | 111865 * | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 99.3 * | 96.8 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 16 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 10 | 1 | reservoir | HEPES | 100 (mM) | |
| 11 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | drop | Brij-20 | 0.01 (%) | |
| 7 | 1 | drop | D-Orn | 15 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 20 (%) | |
| 9 | 1 | reservoir | 0.2 (M) |
