1F3O
Crystal structure of MJ0796 ATP-binding cassette
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2000-04-20 |
| Detector | BRANDEIS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.210, 65.200, 126.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.204 * |
| Rwork | 0.204 |
| R-free | 0.27700 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.146 | |
| Number of reflections | 24520 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 93.5 | 74.5 |
| Redundancy | 4.42 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 294 | PEG 4000, Ammonium Acetate, Glycerol, ADP, Magnesium Chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | MgADP | 10 (mM) | |
| 3 | 1 | drop | protein | 2 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 12 (%) | |
| 5 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | HEPES | 100 (mM) |
