1F3J
HISTOCOMPATIBILITY ANTIGEN I-AG7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 109.540, 109.540, 176.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.820 - 3.100 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iak |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.129 | 0.345 |
| Total number of observations | 102791 * | |
| Number of reflections | 18859 | 3524 * |
| <I/σ(I)> | 6 | 2.3 |
| Completeness [%] | 93.7 | 98.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 20 * | pH 4.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG5000 MME | 10 (%) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 3 | 1 | reservoir | ethylene glycol | 10 (%) | |
| 4 | 1 | drop | HEPES | 20 (mM) | |
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
