1F3H

X-RAY CRYSTAL STRUCTURE OF THE HUMAN ANTI-APOPTOTIC PROTEIN SURVIVIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-14
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	114.040, 71.450, 86.630
Unit cell angles	90.00, 133.37, 90.00

Refinement procedure

Resolution	54.120 - 2.580
R-factor	0.228
Rwork	0.228
R-free	0.28700
RMSD bond length	0.025
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.120	2.640
High resolution limit [Å]	2.580	2.580
Rmerge	0.036 *	0.349 *
Total number of observations	29469 *
Number of reflections	15366 *
Completeness [%]	95.5 *	92 *
Redundancy		3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	sodium-HEPES	100 (mM)	pH7.5
3	1	reservoir	PEG8000	6-8 (%)
4	1	reservoir		200 (mM)
5	1	reservoir	dithiothreitol	2 (mM)