1F3G
THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC
Experimental procedure
Spacegroup name | H 3 |
Unit cell lengths | 80.300, 80.300, 86.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 * - 2.100 |
R-factor | 0.162 |
RMSD bond length | 0.020 |
RMSD bond angle | 3.200 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.100 * |
Rmerge | 0.021 * |
Total number of observations | 15038 * |
Number of reflections | 8279 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | drop | PEG8000 | 20 (%(w/w)) | precipitant |
4 | 1 | drop | sodium acetate | 400 (mM) | precipitant |
5 | 1 | drop | cacodylate | 100 (mM) | precipitant |
6 | 1 | reservoir | precipitant | 1ml |