1F39
CRYSTAL STRUCTURE OF THE LAMBDA REPRESSOR C-TERMINAL DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-11 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.014, 101.335, 114.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.660 - 1.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.105 |
| Total number of observations | 71961 * | |
| Number of reflections | 22468 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 89.1 | 58.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.4 * | 295 | 1.2 M NaH2PO4, 1.05 M KH2PO4, 0.075 M hepes, 25% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 3 (mM) | |
| 5 | 1 | reservoir | 1.2 (M) | ||
| 6 | 1 | reservoir | 1.05 (M) | ||
| 7 | 1 | reservoir | HEPES | 0.075 (M) | |
| 8 | 1 | reservoir | glycerol | 25 (%) |
