1F37
STRUCTURE OF A THIOREDOXIN-LIKE [2FE-2S] FERREDOXIN FROM AQUIFEX AEOLICUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 1999-06-26 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.079, 68.079, 102.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 2.300 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.27000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.530 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.055 | 0.277 |
Total number of observations | 74834 * | |
Number of reflections | 11218 | |
<I/σ(I)> | 24.8 | |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | drop consists of equal volume of protein and reservoir solutions * |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | drop consists of equal volume of protein and reservoir solutions * |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | drop consists of equal volume of protein and reservoir solutions * |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | reservoir | p-dioxane | 4-5 (%(v/v)) | |
6 | 1 | reservoir | MES | 100 (mM) |