1F36
THE CRYSTAL STRUCTURE OF FIS MUTANT K36E REVEALS THAT THE TRANSACTIVATION REGION OF THE FIS PROTEIN CONTAINS EXTENDED MOBILE BETA-HAIRPIN ARMS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07-16 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 51.900, 51.900, 294.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.650 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ORTHORHOMBIC CRYSTAL FORM OF THE LYS36-GLU FIS STRUCTURE |
RMSD bond length | 0.011 |
RMSD bond angle | 23.720 * |
Data reduction software | MSC |
Data scaling software | MSC |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.069 | 0.301 |
Total number of observations | 79532 * | |
Number of reflections | 7139 | |
<I/σ(I)> | 1.63 | |
Completeness [%] | 93.0 | 55.6 |
Redundancy | 11.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | CRYSTALS GROWN BY HANGING DROP VAPOR DIFFUSION FROM A SOLUTION OF 20 MG/ML PROTEIN, 500 MM NACL, 10 MM TRIS-HCL, PH 8.2, 2 M NAFORMATE, AGAINST 4 M NAFORMATE AS THE RESERVOIR, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | sodium formate | 2 (M) | |
3 | 1 | reservoir | sodium formate | 4 (M) |