1F33

THE STRUCTURAL BASIS FOR DNA PROTECTION BY E. COLI DPS PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1997-07-22
Detector	MACSCIENCE
Spacegroup name	C 2 2 21
Unit cell lengths	117.961, 139.123, 263.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 2.600
R-factor	0.209
Rwork	0.209
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DPS D75C D78A UNPUBLISHED STRUCTURE
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.3)
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.590
Rmerge	0.076	0.291
Number of reflections	75305
<I/σ(I)>	8.2
Completeness [%]	98.2	99.7
Redundancy	3.63	3.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K