1F2U

Crystal Structure of RAD50 ABC-ATPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.900, 82.600, 106.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100 *
R-factor	0.238
Rwork	0.238
R-free	0.28200
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.054	0.302
Total number of observations	444072 *
Number of reflections	41804 *
<I/σ(I)>	11.6
Completeness [%]	81.9	51.8
Redundancy	3.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M HEPES, 17% PEG 3350, 8.5% isopropanol, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ATP	2.5 (mM)
2	1	drop	AMP-PNP	2.5 (mM)
3	1	drop		10 (mM)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir	PEG3350	17 (%)
6	1	reservoir	isopropanol	8.5 (%)