1F2U
Crystal Structure of RAD50 ABC-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.900, 82.600, 106.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 * |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.302 |
| Total number of observations | 444072 * | |
| Number of reflections | 41804 * | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 81.9 | 51.8 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, 17% PEG 3350, 8.5% isopropanol, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ATP | 2.5 (mM) | |
| 2 | 1 | drop | AMP-PNP | 2.5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | PEG3350 | 17 (%) | |
| 6 | 1 | reservoir | isopropanol | 8.5 (%) |
