1F2N
RICE YELLOW MOTTLE VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-23 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 283.500, 401.800, 284.000 |
| Unit cell angles | 90.00, 89.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.227 |
| Rwork | 0.218 |
| R-free | 0.21900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.257 |
| Number of reflections | 739272 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 48.7 | 2.8 |
| Redundancy | 1.9 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4 * | 293 | 3.6% (w/v) PEG 8000, 200 mM lithium sulfate, 50 mM sodium citrate, pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 36 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | lithium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 5 | 1 | reservoir | lithium sulfate | 200 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 3.6 (%(w/v)) |
