1F2F

SRC SH2 THREF1TRP MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	277
Detector technology	STORAGE PHOSPHORS
Collection date	1996-06-09
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	74.460, 27.220, 55.250
Unit cell angles	90.00, 106.68, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.208
Rwork	0.208
R-free	0.24000
RMSD bond length	0.012
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.076	0.283
Total number of observations	43530 *
Number of reflections	11664
<I/σ(I)>	23.5
Completeness [%]	97.6	99.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277 *	PEG 4000, sodium acetate,sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)