1F2F
SRC SH2 THREF1TRP MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 277 |
| Detector technology | STORAGE PHOSPHORS |
| Collection date | 1996-06-09 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.460, 27.220, 55.250 |
| Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24000 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.283 |
| Total number of observations | 43530 * | |
| Number of reflections | 11664 | |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 97.6 | 99.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 * | PEG 4000, sodium acetate,sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
