1F27
CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-09-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97186, 0.97954, 0.97969, 0.98743 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 47.590, 30.960, 62.110 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 17.180 - 1.300 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23800 |
RMSD bond length | 0.016 * |
RMSD bond angle | 1.770 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 1.380 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.048 | 0.380 |
Total number of observations | 130824 * | |
Number of reflections | 20852 * | |
<I/σ(I)> | 9 | |
Completeness [%] | 92.0 | 66.4 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 28 * | MPD, POTASIUM HEPES, MGCL2, KCL, BIOTIN, SPERMINE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 28 * | MPD, POTASIUM HEPES, MGCL2, KCL, BIOTIN, SPERMINE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | POTASIUM HEPES | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | KCL | ||
4 | 1 | 1 | BIOTIN | ||
5 | 1 | 1 | SPERMINE | ||
6 | 1 | 1 | MPD | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 1 (mg/ml) | |
2 | 1 | drop | MPD | 3.3 (%) | |
3 | 1 | drop | potassium caodylate | 13.3 (mM) | |
4 | 1 | drop | potassium HEPES | 6.6 (mM) | |
5 | 1 | drop | spermine | 0.1 (mM) | |
6 | 1 | drop | 3.5 (mM) | ||
7 | 1 | drop | biotin | 3.5 (mM) | |
8 | 1 | reservoir | MPD | 22.5 (%) |