1F23

CONTRIBUTION OF A BURIED HYDROGEN BOND TO HIV-1 ENVELOPE GLYCOPROTEIN STRUCTURE AND FUNCTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1999-09-24
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1
Unit cell lengths	38.920, 41.870, 55.980
Unit cell angles	90.59, 88.93, 96.28

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.191 *
Rwork	0.191
R-free	0.27000
RMSD bond length	0.006
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.076	0.287
Total number of observations	62249 *
Number of reflections	14667
<I/σ(I)>	7.7
Completeness [%]	96.1	92.8
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	Sodium citrate, PEG 4000, propanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	propanol	5 (%)
4	1	reservoir	PEG4000	7 (%)