1F21

DIVALENT METAL COFACTOR BINDING IN THE KINETIC FOLDING TRAJECTORY OF E. COLI RIBONUCLEASE HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	37.270, 40.830, 85.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.400
Rwork	0.217
R-free	0.25400
RMSD bond length	0.006 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.063 *	0.136
Number of reflections	24663
<I/σ(I)>	16.7
Completeness [%]	92.8	59.8 *
Redundancy	21.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	Hepes, pH 7.5, PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-10 (mg/ml)
2	1	reservoir	HEPES	20 (mM)
3	1	reservoir	PEG3350	5-15 (%)