1F1Z

TNSA, a catalytic component of the TN7 transposition system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1998-10-02
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	38.350, 83.680, 97.940
Unit cell angles	90.00, 94.07, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.202
Rwork	0.202
R-free	0.25400
RMSD bond length	0.009
RMSD bond angle	1.676
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.197
Total number of observations	131670 *
Number of reflections	23596 *
<I/σ(I)>	13.2
Completeness [%]	97.0	80.7
Redundancy	5.58	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	PEG 8000, Isopropanol, MgCl2, NaCl, Tris-HCl HEPES, Triton X100, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-8 (mg/ml)
10	1	buffer 1	PEG8000	10 (%)
11	1	buffer 1	Tris-HCl	0.1 (M)
12	1	buffer 1		50 (mM)
13	1	reservoir		0.1 (M)
14	1	reservoir	isopropanol	10 (%)
15	1	reservoir	buffer 1		90 %
2	1	drop	HEPES	25 (mM)
3	1	drop		0.5 (M)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	2 (mM)
6	1	drop	Tritron X-100	0.01 (%(v/v))
7	1	drop	glycerol	10 (%(w/v))
8	1	drop	isopropanol	10 (%(v/v))
9	1	drop	buffer 1