1F1V
ANAEROBIC SUBSTRATE COMPLEX OF HOMOPROTOCATECHUATE 2,3-DIOXYGENASE FROM ARTHROBACTER GLOBIFORMIS. (COMPLEX WITH 3,4-DIHYDROXYPHENYLACETATE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-02 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.420, 59.300, 103.200 |
Unit cell angles | 90.00, 118.39, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.166 |
Rwork | 0.166 |
R-free | 0.20000 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.900 * |
Data reduction software | CRYSTAL (CLEAR) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.236 * |
Number of reflections | 56746 | |
<I/σ(I)> | 15.5 | |
Completeness [%] | 96.7 * | 93.6 * |
Redundancy | 3.80 * | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 * | 18 * | Peg 8000, Mg Acetate, Na cacodylate, pH 6.8, Batch crystallization, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 100 (mg/ml) | |
2 | 1 | 2 | PEG8000 | 6-10 (%) | |
3 | 1 | 2 | magnesium acetate | 0.2 (M) | |
4 | 1 | 2 | sodium cacodylate | 100 (mM) | pH6.5 |