1F1S
CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE AT 2.1 ANGSTROM RESOLUTION.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-15 |
| Detector | BRANDEIS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.265, 156.765, 237.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.191 * |
| Rwork | 0.191 |
| R-free | 0.25300 |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.145 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 * | 0.172 * |
| Total number of observations | 484052 * | |
| Number of reflections | 54880 | |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 97.0 | 77.2 * |
| Redundancy | 3.59 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | Jedrzejas, M.J., (2000) Acta Crystallogr., Sect.D, 56, 460. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylate | 100 (mM) | pH6.0 |
| 2 | 1 | reservoir | KSCN | 30 (mM) | |
| 3 | 1 | reservoir | PEG5000 MME | 19 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | HEPES | 10 (mM) | |
| 6 | 1 | drop | EDTA | 2 (mM) | pH8.0 |
