1F1O
STRUCTURAL STUDIES OF ADENYLOSUCCINATE LYASES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 129.530, 129.530, 282.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| Rwork | 0.336 |
| R-free | 0.35500 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.250 |
| Rmerge | 0.058 |
| Total number of observations | 22850 * |
| Number of reflections | 2843 |
| Completeness [%] | 87.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 18 * | This particular structure is not described in this paper. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | citric acid | 0.1 (M) | pH5.0 |
| 2 | 1 | reservoir | PEG4000 | 10-20 (%(w/v)) | |
| 3 | 1 | reservoir | 0.02 (%(w/v)) |
