1F1M
CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN C (OSPC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-23 |
Detector | BRANDEIS - B1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.323, 46.252, 111.784 |
Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.24000 * |
RMSD bond length | 0.004 |
RMSD bond angle | 1.010 |
Data reduction software | MARMAD |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.087 | 0.216 |
Number of reflections | 531522 * | |
Completeness [%] | 85.3 | 21.6 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG MME 550, MES, Zinc sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.9 (mg/ml) | |
2 | 1 | reservoir | mPEG550 | 25 (%(v/v)) | |
3 | 1 | reservoir | zinc sulfate heptahydrate | 10 (mM) | |
4 | 1 | reservoir | MES | 100 (mM) |