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1F1M

CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN C (OSPC)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X12C
Synchrotron siteNSLS
BeamlineX12C
Temperature [K]100
Detector technologyCCD
Collection date2000-02-23
DetectorBRANDEIS - B1
Spacegroup nameP 1 21 1
Unit cell lengths66.323, 46.252, 111.784
Unit cell angles90.00, 99.08, 90.00
Refinement procedure
Resolution50.000 - 1.800
R-factor0.201
Rwork0.201
R-free0.24000

*

RMSD bond length0.004
RMSD bond angle1.010
Data reduction softwareMARMAD
Data scaling softwareSCALEPACK
Phasing softwarePHASES
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.840
High resolution limit [Å]1.8001.800
Rmerge0.0870.216
Number of reflections531522

*

Completeness [%]85.321.6
Redundancy44
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.5293PEG MME 550, MES, Zinc sulphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein6.9 (mg/ml)
21reservoirmPEG55025 (%(v/v))
31reservoirzinc sulfate heptahydrate10 (mM)
41reservoirMES100 (mM)

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PDB entries from 2024-11-06

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