1F18
Crystal structure of yeast copper-zinc superoxide dismutase mutant GLY85ARG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.500, 118.500, 73.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 1.700 |
| Rwork | 0.197 |
| R-free | 0.23400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.850 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | |
| Number of reflections | 17737 | |
| Completeness [%] | 80.1 | 51.3 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 275 | Ammonium Sulfate, Tris-HCl, NaCl, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 275K |
