1F15
CUCUMBER MOSAIC VIRUS (STRAIN FNY)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 336.000, 336.000, 336.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.247 * |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 3.100 |
| High resolution limit [Å] | 3.000 * | 3.000 |
| Rmerge | 0.046 * | 0.131 |
| Completeness [%] | 57.0 * | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | Sodium formate, sodium acetate, PEG 8000, cyclohexyl-pentyl-D-maltoside, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 2 (M) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | PEG8000 | 0.05-0.125 (%) | |
| 4 | 1 | drop | sodium formate | 1 (M) | |
| 5 | 1 | drop | sodium acetate | 0.05 (M) | |
| 6 | 1 | drop | PEG8000 | 0.025-0.0625 (%) | |
| 7 | 1 | drop | Tris | 4 (mM) | |
| 8 | 1 | drop | protein | 0.8 (mg/ml) | |
| 9 | 1 | drop | CYMAL-5 | 2.4 (mM) |
