1F0X

CRYSTAL STRUCTURE OF D-LACTATE DEHYDROGENASE, A PERIPHERAL MEMBRANE RESPIRATORY ENZYME.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	200
Detector technology	CCD
Collection date	1999-06-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97851, 0.97880, 0.97116
Spacegroup name	P 1 21 1
Unit cell lengths	69.006, 74.187, 101.997
Unit cell angles	90.00, 95.73, 90.00

Refinement procedure

Resolution	20.000 * - 1.900
R-factor	0.201 *
Rwork	0.209
R-free	0.24500 *
RMSD bond length	0.024
RMSD bond angle	2.430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.030
High resolution limit [Å]	1.900	1.900
Rmerge	0.081 *	0.267
Total number of observations	1154879 *
Number of reflections	95767 *
<I/σ(I)>	9.1
Completeness [%]	91.1 *	96.1
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	PEG 4000, potassium phosphate, mercaptoethanol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	3-5 (%(w/v))
2	1	reservoir	sodium acetate	0.1 (M)