1F0T
BOVINE TRYPSIN COMPLEXED WITH RPR131247
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-02 |
| Detector | MACSCIENCE DIP100S |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.440, 63.830, 69.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.900* |
| R-factor | 0.172 * |
| Rwork | 0.179 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 * | 0.165 * |
| Number of reflections | 21256 * | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 93.0 * | 91.7 * |
| Redundancy | 3.5 | 3.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 19 * | 1.85-1.9M ammonium sulfate, 50 mM Mes-NaOH pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES-NaOH | 50 (mM) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | benzamidine | 60 (mM) | |
| 4 | 1 | drop | protein | 30 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.85-1.90 (M) | |
| 6 | 1 | reservoir | MES- NaOH | 50 (mM) |
